3

While TTP measurements between 25 days and the diagnostic LOD may be important for diagnostic purposes, TTP values in this range may not contribute meaningfully to its use as a quantitative measure, particularly when assessing treatment response, and may lead to under-powered clinical trials.

Human and mosquito movement can lead to underestimation of the intervention effect in trials of vector interventions; as such, the protective efficacy of Wolbachia is likely even higher than reported in the primary trial results.